With Dr. PRP Platelet separation kit, it’s easy to collect your patient’s blood for the purpose of extracting Platelet-Rich Plasma. Dr.PRP uses a simple, unique design to efficiently assist physicians or lab technicians in the preparation.
The blood samples taken from the patient are transferred to Dr. PRP kit for Platelet separation using a centrifuge. The final components of the blood will be the red blood cells, the platelets, and the plasma.
When platelets become activated, growth factors are released into the area surrounding the wound or injury that the patient is treated for.
High Platelet Recovery Rate
Platelet concentration determines the efficiency of a good PRP separation system regardless of the volume of Platelet Rich Plasma (PRP) produced. Dr. PRP uses a specially designed tube and a recommended centrifuge speed to help you get the perfect Platelet-Rich Plasma concentration for your patient’s treatment needs.
PDGF and all it’s isoforms (AA, AB, BB, CC, DD) are a family of peptides associated with formation of connective tissues, glial and muscle cells.
Transforming Growth Factor (TGF-ß1, TGF-ß2)
– Promotes formation of extracellular matrix
– Regulates bone cellmetabolism
Vascular Endothelial Growth Factor (VEGF)
– Promotes angiogenesis
Epidermal Growth Factor (EGF)
– Promotes cell differentiation and stimulates
– Re-epithelialisation, angiogenesis and collagenase activity
Fibroblast Growth Factor (FGF)
– Promotes proliferation of endothelial cells and fibroblasts
– Stimulation of angiogenesis
The higher the platelet recovery, the more concentrated the growth factors in the resulting samples which can be used for a variety of treatments.”
How To Use Dr. PRP Platelet-Rich Plasma Kit
1STSTEP
In a 20 cc syringe take 2 cc of anticoagulant, then draw 18cc of blood from a subject (final volme : 20 cc).
2NDSTEP
Inject the drawn blood to Dr.PRP kit through the upper injection port until the blood level reaches the 20cc scale marked on the kit. (For easier injection, tilt the kit)
3RDSTEP
- Separate the plasma layer and red blood cell (RBC) layer by the first centrifugation. (Prior to centrifugation, balance the weight of kit with counterbalance)
- Conditions for the First Centrifugation (RPM)
Female - 3,000 RPM 3 minutes
Male - 3,000 RPM 4 minutes
When fail the seperation - 3,000 RPM 1 minutes
*For subjects taking aspirin, antihypertensive drugs, with cardiac disease, anemia, or during menstruation, the centrifugation should be done for 4 min at 1,000 RCF.
4THSTEP
After the first centrifugation, identify the separation position of the plasma and the RBC layer. Then adjust the height of the separated boundary to the indicated point by pushing up or pulling down the adjusting knob located at the lower part of the Kit. (If the volume of the plasma layer or the separation is not quite well done, additional centrifugation may be done for about 1-2 min at same RPM)
5THSTEP
In order to block the plasma and the RBC layer completely, fasten the adjusting knob (counter-clockwise) located at the lower part of the Kit. Then fasten the valve(counter-clockwise) just above the adjusting knob. Finally fasten the adjusting knob(counter-clockwise) again.
6THSTEP
Put the fastened PRP Kit to the centrifuge with counterbalance for the second centrifugation to enrich the concentrated platelet.
*Condition for the second centrifugation : 6 min at 3,200 RPM
7THSTEP
Place the PRP Kit in its upright position with care in order to prevent mixing of the separated PRP and PPP. Open the upper sillicone lid on the Kit. Using a 10cc syringe with a long needle (> 5 cm) take out the PPP layer slowly from the upper part leaving 4 cc at the base.
8THSTEP
Shake and mix the remaining 4 cc of the PRP layer. Then administrate the PRP.
Dr. PRP Buffy Coat Extraction Process
Click on the button to watch the video so you’ll learn more.
Certified Safe
Dr. PRP is registered with the US-FDA as a blood collection tube and is manufactured under ISO-certified process enabling it to be used by the following medical professionals and facilities:
Veterinarians
Orthopedic Doctors
Sports Medicine Clinics
Plastic Surgeons
Dermatologists
Orthopedic Surgery Centers
A PRP Therapy For Hair Restoration In Progress. Click on the images to watch the video.
Dr. PRP buffy coat extraction can be done in a single step. For further higher concentration, a second step is needed.
1 In the first centrifugation spin, yellowish layer of plasma and the buffy coat layer of leukocyte and platelets are separated. This separated content may also contain some Red Blood Cells.
2 In the second centrifugation spin, the lower fraction Platelet Rich Plasma and White Cells are extracted.
